Delivery of superoxide dismutase by TAT and Abalone peptides for the protection of skin cells against oxidative stress

Trichoderma reesei superoxide dismutase (TrSOD) is a well characterized enzyme being stable between 30 and 90°C for 1 h with activity at pH between 2.6 and 9.0. This work aimed to clone, express, purify and evaluate the protective effect antioxidant of this enzyme on skin cells when fused to transactivator of transcription (TAT) protein transduction domain of HIV-1 and Abalone (Ab) peptides to allow cell penetration. TrSOD, TAT-TrSOD-Yfp (fused to yellow fluorescent protein) and Ab-TrSOD were expressed in E.coli and purified as soluble proteins. The cytotoxicity of the enzymes, at the concentrations of 1, 3 and 6 μmol/L, was evaluated for a…
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A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping

Background: The functional understanding of genetic interaction networks and cellular mechanisms governing health and disease requires the dissection, and multifaceted study, of discrete cell subtypes in developing and adult animal models. Recombinase-driven expression of transgenic effector alleles represents a significant and powerful approach to delineate cell populations for functional, molecular, and anatomical studies. In addition to single recombinase systems, the expression of two recombinases in distinct, but partially overlapping, populations allows for more defined target expression. Although the application of this method is becoming increasingly popular, its experimental implementation has been broadly restricted to manipulations of a limited set of common…
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development of novel therapies for pediatric cancer

development of novel therapies for pediatric cancer

DESCRIPTION (offered by applicant): Pediatric cancers are orphan malignancies that happen in sufferers who've traditionally been missed for essentially the most half by the pharmaceutical and biotechnology industries; thus, the pediatric cancers could be thought-about double orphans" - orphan ailments that happen in orphan sufferers. Although dramatic strides have been achieved over the previous 40 years within the administration of the pediatric cancers, there stay many shortcomings within the efficacies and toxicities of accessible therapies for the varied varieties of childhood malignancy. development of novel therapies for pediatric cancer The main objective of this utility is to facilitate the development of new applied…
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neurokinin-1r (sp receptor) antagonists for hiv therapy

neurokinin-1r (sp receptor) antagonists for hiv therapy

DESCRIPTION (provided by applicant): New HIV-1 therapies that utilize novel antiviral mechanisms and exert a positive immunomodulatory effect are needed. Neurokinin-1 receptor (NK-1R) antagonists target the substance P (SP) receptor and have demonstrated antiviral and immunomodulatory effects. neurokinin-1r (sp receptor) antagonists for hiv therapy The NK-1R antagonists are a new therapeutic target with the potential to interrupt a pathway critical to HIV replication. The goal of this Integrated Preclinical/Clinical Program (IPCP) is to identify an NK-1R antagonist that is: (a) active as an anti-HIV agent through interaction with chemokine/cytokine receptors (Project 1); (b) specific for NK-1R interaction with CCR-5 (Project…
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Distinct Developmental Features of Olfactory Bulb Interneurons

Distinct Developmental Features of Olfactory Bulb Interneurons

ABSTRACT The olfactory bulb (OB) has an extremely higher proportion of interneurons innervating excitatory neurons than other brain regions, which is evolutionally conserved across species. Despite the abundance of OB interneurons, little is known about the diversification and physiological functions of OB interneurons compared to cortical interneurons. In this review, an overview of the general developmental process of interneurons from the angles of the spatial and temporal specifications was presented. Then, the distinct features shown exclusively in OB interneurons development and molecular machinery recently identified were discussed. Finally, we proposed an evolutionary meaning for the diversity of OB interneurons. INTRODUCTION…
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Do the Walkability and Urban Leisure Amenities of Neighborhoods Affect the Body Mass Index of Individuals? Based on a Case Study in Seoul, South Korea.

Do the Walkability and Urban Leisure Amenities of Neighborhoods Affect the Body Mass Index of Individuals? Based on a Case Study in Seoul, South Korea.

This study investigates the impact of neighborhood-built environments on obesity in interrelationship with socioeconomic status (SES)-controlling for dietary patterns and physical activities of residents-using structural equation modeling (SEM). A total of 577 samples who are between 19 and 64 years old and reside in Seoul are extracted from Korea National Health and Nutrition Examination Survey (KNHNES), 2015. Neighborhood environments are represented as the two latent constructs-walkability and leisure amenities-composited with indicators such as density of intersections, density of mixed-use area, and the area of open spaces and are aggregated by jurisdictional unit in Seoul. We found that greater walkability in…
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그리드의 규칙적인 2-D 홀의 레이저 회절을 사용하여 Cryo-Electron 현미경 그리드에서 액체 양의 실시간 측정

Cryo-electron microscopy (cryo-EM)는 이제 거대한 단백질 복합체의 고해상도 구조를 결정하는 첫 번째 선택입니다. 탄소 필름으로 덮인 2 차원 구멍 배열을 가진 격자는 일반적으로 cryo-EM에 사용됩니다. 반자동 플런저를 사용할 수 있지만 적절한 그리드 시편을 얻기 위해서는 여전히 시행 착오가 필요합니다. 여기, cryo-EM 그리드에서 액체 양의 실시간 측정을 사용하여 얇은 얼음 표본을 얻는 새로운 방법을 소개합니다. cryo-EM에 대한 그리드는 레이저 광을 강하게 회절 시켰으며, 각 스폿의 회절 강도는 실시간으로 측정 가능했다. 측정 된 회절 패턴은 주위의 액체의 곡률로 인해 구멍 내의 액체의 상태를 나타냈다. 회절 패턴을 사용하여, cryo-EM의 그리드를 동결시키기위한 최적의 시점을 실시간으로 얻었다. 이 개발은 연구원들이 제한된 cryo-EM 기기 접근 자원을…
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구조 기반 약물 재배치에 의한 이노시톨 폴리 포스페이트 멀티 키나제의 억제제로서 항우울제 Vilazodone의 식별

이노시톨 폴리 포스페이트 멀티 키나제 (IPMK)는 IP3 및 IP4와 같은 다중 IP 대사 산물의 인산화를 통해 이노시톨 포스페이트 (IP)의 생합성을 위해 요구된다. 성장 및 신진 대사와 같은 세포 신호 전달 사건을 조절하기위한 IPMK의 촉매 작용의 생물학적 중요성이 광범위하게 연구되었다. 그러나 IPMK를 억제하는 약리학 적 시약은 아직 밝혀지지 않았다. 우리는 IPMK 억제제로서 항우울제 인 빌라 조돈을 확인한 공개적으로 이용 가능한 미국 식품의 약국 승인 의약품 및 화학 물질의 구조 기반 가상 스크리닝을 사용했습니다. 도킹 시뮬레이션 및 약전 분석은 vilazodone이 ATP보다 IPMK의 ATP 결합 촉매 영역에 대해 더 높은 친화력을 가지고 있으며 vilazodone이 시험관 내에서 IPMK의 IP 키나제 활동을 억제 함을 검증했습니다.…
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Exploring the Determinants of the Severity of Pedestrian Injuries by Pedestrian Age: A Case Study of Daegu Metropolitan City, South Korea.

Exploring the Determinants of the Severity of Pedestrian Injuries by Pedestrian Age: A Case Study of Daegu Metropolitan City, South Korea.

Pedestrian-vehicle crashes can result in serious injury to pedestrians, who are exposed to danger when in close proximity to moving vehicles. Furthermore, these injuries can be considerably serious and even lead to death in a manner that varies depending on the pedestrian's age. This is because the pedestrian's physical characteristics and behaviors, particularly in relation to roads with moving vehicles, differ depending on the pedestrian's age. This study examines the determinants of pedestrian injury severity by pedestrian age using binary logistic regression. Factors in the built environment, such as road characteristics and land use of the places where pedestrian crashes…
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메티 오닐 -tRNA 합성 효소는 초파리의 수명을 조절한다

메티 오닐 -tRNA 합성 효소 (MRS)는 번역에 필수적이다. MRS 돌연변이 체는 전체 번역을 감소 시키며, 이는 일반적으로 다양한 유전자 모델에서 수명을 증가시킨다. 그러나, 우리는 감소 된 단백질 합성에도 불구하고 MRS가 초파리 감소 된 수명을 억제한다는 것을 발견 하였다. MRS 억제 된 초파리를 이용한 Microarray 분석은 염증성 및 면역 반응 유전자의 현저한 변화를 나타냈다. 특히, 항 미생물 펩티드 (AMP) 유전자의 발현이 감소되었다. 우리가 노화 동안 AMP 유전자의 발현 수준을 측정 할 때, 이들은 대조 파리에서 증가하고 있지만 연령에 따라 MRS 억제 파리에서 감소되었다. 흥미롭게도, 무균 상태에서, MRS 억제 파리의 최대 수명은 종래의 상태와 비교하여 증가되었다. 이러한 발견은 초파리에서 하향 조절 된…
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